I keep having the same issue when I attempt to use the manual drawing function to identify objects in Cell Profiler. When I attempt to load the image, the entire image is dark and I an unable to see my cells. Most of the time, the image is completely black but sometimes I can see a faint image. Any idea what I am doing wrong or what is causing this? I have tried this with and without adding the Illumination correction module. Thanks in advance to any response!