I’m new to cellprofiler and I am having some diffuclities.
I need to count cells, and I have something like 2000 cells in the pic.
The problem is that part of the pic the identifyprimary doesn’t identify the cell because they are very bright,
so it can identify only part of the cells. How can i make part of hte pic that is bright because of the kight darker?
so it will look like the rest of the pic? and that i can identify all of the cells in the pic?
I added the segmention that i’m getting.
I would really appreciated any help.
problem.pdf (424 KB)