I want cellprofiler to find the cells in the attached image. I have tried to do this using IdentifyPrimaryObjects. However it struggles due to the cells having irregular shape and staining. Several of the cells might have more than one nuclei, and I therefore do not think using the nuclei as primaryObject and the cells as secondaryObject would work. What do you think is the best way to find all the cell objects in this image?
Thanks in advance,
WGA_small.tif (9.0 MB)