Is it possible to identify 2nd objects using 2 markers? I have Phalloidin channel which is sometimes weak, and another channel that sometimes works pretty well. Occationally they stain whole cell mutually exclusively good - if you know what I mean. I assumed there is no option of using more than 1 marker in identifying objects module. Hence, I try to manipulate the masks instead. Two of 2nd objects - say phalloidin and, say, m2 are saved. From my observation, a simple union operation of phalloidin and, say 2nd marker, m2 might just do the work. I am wondering if there is a way to do so, and then extract features from that manipulated mask instead. Otherwise, I can process this outside cellprofiler, then I am wondering if there is a way to import the mask back.
Any suggestion would be greatly appreciated. Thank you for such an awesome tool and great forum.