I am new to ICY (started working with it after the excellent webinar in the NEUBIAS home academy) and I am trying to set up a workflow to detect and track spots over time in 3D.
Is that possible with the “Spot Detector” + “Spot Tracking” plug-ins in ICY?
I was able to detect spots and load them into the tracking module, but I am not sure if the results I got were calculated in 3D. The input is a single channel z-stack over 6 time points; I applied a Median 3D and a LocalMaximum Filter:
Thanks in advance!!