Icy spot detection with noise background

Hi everyone,

I am trying to analyse some acquisitions with Spot Detector on Icy, in order to Detect some bacteria in time. However, I used two markers emitting at two different wavelengths, unfortunately, one on them induces a noise background (diffuse background + spots) when I look at the signal of the second marker.

I was wondering if there was a plugin on Icy which enables to convert this noisy signal to a mask that I could substract from my signal of interest. I don’t know if it is clear.

Thanks in advance for your help!

Thomas