I’m new to image data processing and especially to Icy. I was wondering why is it that, even though the ROIs on the ROI tab appear as having integer pixel coordinates, when I try to export them to excel I obtain positions that have decimal precision. I’m working with polygon contour ROIs (from the Active Contours plugin).
Because I’ll be studying the trajectory of each ROI (which represents a nucleus) on a different platform, it’s important that I understand how the center coordinates (Center X and Center Y) of each ROI are calculated.
Thank you advance for your help! I couldn’t find the answer to this question anywhere.