I would like to quantify how much fribronectin and its orietnation, the cells laid down in respect with Phalloidin

<a class="attachment" href="/uploads/cellprofiler/original/2X/7/7861fa7131e9b33983627194b5fd171d743773ba.cpproj">fibro1.cpproj</a> (937.2 KB)

I could not find a threshold which can take into account all the fibronection laid down from the cells…I have tried different threshold…

Do you mind attaching the .cpproj file and example images? Your initial attempt to upload seems to have failed.

Dear Anne,

Thank you very much for your help.

I would like to be able to quantify the amount of the fibronectin in red or the second channel image in the stack. I have attached two pictures because I have to quantify the amount of fibronectin laid down on the soft and stiff gels (stack 11-soft and 1-stiff gel).

Best regards,
Oana Fibronectin program.cpproj (974.4 KB)

Your images still aren’t uploading, sorry.