I need to characterise these cells, nucleus, cytoplasm and n

I installed the CellProfiler 2.0 and I would like to obtain maximum of information from the files attached. These files with digital images are attached.
I need to characterise these cells, nucleus, cytoplasm and nucleolus.
Thank you in advance, Stefan











Hi,

These look to be electron microscopy images. CellProfiler natively works best with fluorescent images, so I would suggest using Ilastik, which is a pixel classifier that now comes bundled with CellProfiler. Try that out first, independent of CP, then you can use the ClassifyPixels module in CP to input the classification results. They have some instructions here.

Let us know how it goes!
David