I need suggestions to solve a segmentation problem.
The propose is to count fibres that cross the intradermal layer as suggested in figure result. I was first trying to segment the intradermal using either ch2 (dapi) or ch3 background and then find the maximum values to identify the fibres in channel 1.
The problem is with the segmentation od intradermal layer. Do you have any suggestion that could be generalized for other similar images?
I also have the option of stitching all the images of the same biopsy in order to better identify the intradermal, but it turns to be much heavy for processing
Thank you in advance.
Mafaldaresult.tif (3.0 MB)