I am new to imagej.
I took several images from grey particles covering a green surface.
I d like to compare those images for particle coverage.
The images were taken with suboptimal lighting conditions.
That means the brightness is not equal in the image.
This appears to hinder my analysis to some degree: very subtle particle coverage issues are not detectable.
I want to give an example:
Here is one image with some coverage of grey particles.
Two things stand out here:
- At the bottom left area, the green surface is visible through the particles (green indication). The remaining area seems covered.
- The left side is brighter than the right side of the image. the middle seems to be reddish.
When I use a threshold on the 8 bit Version I get the following binary result.
The bottom left area (yellow indication) is detected as “not perfectly covered” but also -unwantedly- the right area of the image (yellow indication).
Is there anyone out there who might know what to do to obtain a threshold which is sensible to the green surface and not so much to the overall brightness of the image after conversion to 8-bit.
The raw data is a png.
I tried using 8-32 bit, Lab and HSB stack types with the same issue.
I tried the 3D color inspector but couldn’t find any useful depth/rotation setting.
Any help apprechiated.