I am working on a pipeline identifying cytoplasmic speckles.
In the end I want to derive a “speckles per cell” ratio and therefore I need to relate the child objects “speckles” as accurately as possible to the parent objects “cytoplasm”. I have some difficulties identifying the cells itself. No matter which settings I use, the labeled image is not what I want to see.
I attached some screenshots to my post to clarify what I mean.
I would appreciate any help! Thank you!