I have some single channel fluorescence image data and try to use ilastik. When I open it, the image is too dark to work on. I tried to change the contrast by change the range. But I cannot change the min above 99. Besides, even I lowered the max to less than 1000, the image does not seem to change at all. The raw data is tif so I converted the image to png and encountered the same issue. please help. Thank you
would you mind sharing this image? (You can do so non-publicly via firstname.lastname@example.org).
emailed a screen shot to you