I want to use Manual Tracking with Trackmate of objects in a 3D space. I know this plugin is used for tracking over time, but I cannot find another plugin that allows for manual labeling of such complex structures. Although it does everything I need it to do, I can only use it to analyse few images as my images are very big. Therefore, I would like to create a hyperstack in which each z slice is associated with only one artifical time frame (t).
Let’s say I have 50 z slices, for each of those slices I would want one artificial timepoint so the plugin can store the links between particles, and at the same time give a 3D representation in the end.
Currently that would require to create a hyperstack with 50 z slices, and 50 associated t frames, leading to 2500 images, and I don’t have the RAM memory to handle that.
Looking forward to any help,