I measured the intensity statistics for each nuclei. Now, I would like to subtract the mean intensity of each single nuclei from the staining. I thought, I could create an image containing per nuclei the mean intensity value and then use this for subtraction.
But I cannot find a way to do this. Or is there any other possibility to make an object based intensity subtraction?
(I’m working with CP 2.1.1 as it runs on our cluster)
Can you explain your use case a bit more clearly in context? I don’t think there’s a way to make an image with the mean values from each nucleus, but it’s possible that with more information we could help you figure out how to achieve whatever your downstream purpose is.