I think I missed something in using properly classifiers in QuPath.
I have images in brightfield with two stainings. I draw a ROI, I detected cells, and I draw labelled annotations around cells (stain1, stain2 and unstained classes). Then, I used the “create a detection classifier” tool. I chose the features and after a few trials, I was quite happy with the classification of all cells (auto update) of my ROI. So I saved the classifier.
Then, in order to use it on another ROI (close to the first one, with same types of cells and stainings), I loaded the detection classifier. But every cells were labelled as stain1.
Did I missed something in saving the classifier or in applying it ?
(I’m using milestone 8)
Thanks by advance