Hi everyone! I need your help!
My name is Sara, I´m working in telomere (labeled with GFP) movement analysis in yeast cells. To do this I have to follow a point during a time lapse and look for the Z-stack where the point is most focused (my point moves in Z, Y and Z). I use ManualTrack or MTrackJ plugins of ImageJ, but with these plugins I have to manually select my point of interest throughout all time lapse and look for the focused Zstack, for each of my cells. I would like to find one or some plugins to analyze my images faster. For this I need to find a plugin that automatically detects the telomere marked in each cell in the most focused Zstack, draws for me the tracking that follows along the time lapse and measure the average speed at which my point moves and the distance it travels (this measures are automatically obtain with the MTrackJ plugin). I would like to know if someone knows how to do this and what plugins could be use, or maybe explain me how to make a simple macro.
Initially, to try to simplify the process, I thought about doing the maximum projection, but how my point moves faster than time that takes for the microscope to perform the full zstack, sometimes 2 points appear instead of one. So I need a plugin that can detect the point in a zstack.
Also, I have tried to use the “find maximum” (single points) for points detection. It detects points correctly, but only in a frame. I have found a macro to make maximum Find stack, and it seems that it looks for points throught Zstack and time lapse, but after they are not marked in image.
I have also tried using TrackMate, but with this plugin I don´t know how obtain adjusted conditions for automatically select my points.
I don´t have any more ideas!! If someone can help me or suggest me any plugin or explain me how to write a simple macro… it would be great!
I attach an example of my images and a macro that I found.macro.txt (1.3 KB)