I am an undergraduate student that is tracking the movement of cells in a monolayer. I am loving Cell Profiler, but I am having some issues with the TrackObjects module. I have been using Overlap to track cells with maximum distance of matching at 1, but after numerous frames, TrackObjects still has made matches will multiple cells and they have the same identification number. I was wondering how can I set up TrackObjects so that the matches are only made with one other cell (as in the same cell from the previous frame)? Is this possible?
Edit to include figures:
Here is the images I am working with along with my current pipeline: