im a grad student new to imaging quantification.
that being said i would like to track the changes in intensity of each pixel for a region of interest over time.
specifically, i have a 200x200 ROI (40,000 pixels total), and i would like to track how each pixel changes in intensity over time (not the average intensity for the whole square). This is a calcium imaging experiment, so i have increases in signal intensity occurring randomly in processes within this 200x200 square.
Any input or advice as to specific plugins would be greatly appreciated!
thanks so much!!!