Thanks in advance if you offer help, this website has been tremendously helpful in figuring out image processing.
Thanks to you all, I figured out how to keep my 4 channels separate in Image J. However, after stitching together two images, they seem to form 1 RGB image (it will say it has 4 channels, but it’s really just 1- and the colors seem seemed up. For example, DAPI will now look red instead of blue).
I would like to stitch them together, based on just DAPI for example – and then be able to open the stitched image back on ZEN blue, with my four distinct channels intact so I could do my analysis of each channel there.
Can anyone help me figure out how to accomplish that? Or any software recommendations for doing that in an easier way? Thanks!!