NIR Perfusion-2.tif (90.8 KB)
I have a problem with intensity measurements in RGB Images. I get heat map / LUT images with the corresponding scale bar from my medical measurement device (part of it in the attached image, since it is patient data I needed to cut it so don’t wonder why it looks so nonsense).
I now need to measure the intensity in a self defined ROI in this image. Perfect would be if I could set the given LUT scale in image J and use this for measurement of the ROI.
I already tested the following
- it can not be converted from LUT to greyscale intensity since its an RGB and LUT is not created in Image J
- Calibration Bar does not work in my Fiji (newest version) on the RGB image, transfer to 8-bit color results in loss of information and gives crap with the Calibration Bar
- split into 3 channels works and intensity is measured in gray scale (256 steps per channel) but splitted it makes no sense anymore. I added the values, but if only on channel is high I can not distinguish just by values if my measurement is blue / red or whatever.
Has anybody an idea? Or know which tool to use?