How to segment objects in 1 colour from images

Hi forum !

I have some research images of stained histological slices that contain ‘blue blobs’ dispersed around ‘brown circular structures’. I need to segment the large brown circular structures from the blue blobs. Any suggestions please ? I am unable to include an image by example as an error message states that the attachmnet quota has been reached.

regards
bal

Hi,

The forum attachment limits have been increased so you should be able to upload images.

Without seeing the images, I can still offer the following suggestion: I have found that splitting the color image into the individual color channels using ColorToGray, and identifying the blue blobs from the blue channel and segmenting the brown structures using the red channel (which can sometimes be used as a surrogate for the color brown) may prove fruitful.

Regards,
-Mark