How to segment, label and compute features on microvessel density images using ImageJ?

How to segment, label and compute features on microvessel density (lymphangiogenesis) images using ImageJ? What plugins to choose?
Is there a virtual Chalkley method plugin available for download and installation?

You can segment your images with the Trainable Weka Segmentation plugin. The output labels can then be processed using something like MorphoLibJ’s label tools.

thank you, how to use imageJ in the Java code with OpenCV, is there any tutorial?

Yes, have a look at this wiki page about using native libraries from ImageJ code.

I suggest using the Angiogenesis Analyzer. It’s really great for blood vessel analysis if you are looking for length, branches, nodes, etc. It can also be used for neurite outgrowth. There are sample data sets available for testing. As well as phase contrast and fluorescence images, I’ve also successfully analyzed DIC images. If you have brightfield, you can probably invert the LUT and use the fluorescence option.
http://image.bio.methods.free.fr/ImageJ/?Angiogenesis-Analyzer-for-ImageJ
https://imagej.nih.gov/ij/macros/toolsets/

I forgot to say that there is a Chalkley grid plugin available. I think it’s hard to find online but I have it if you want it. It puts a grid over your images in the same way as would have happened with a physical eyepiece grid.

thank you, please post a link 4 download

Hi, I’ve been searching for a digital Chalkley grid plugin and came across your comment! I was wondering if you may be able to point me towards where I can find this?
Thanks in advance!