So I have a stack of images that is saved as an OIB and I’d like to place markings on it (such as arrows using the arrow tool), but I can’t figure out how to save it so that I can open up the same OIB file with the arrows I’ve placed. How can I do this?
I don’t think you can do this directly by resaving as OIB.
What you could do is to annotate your image (overlay, ROI) and then save it as an ImageJ TIFF. ImageJ will be able to retrieve the annotation when you reopen the TIFF image (but not other softwares).