An example tiff image is attached. Some of the grids I see feature folds in the coating. These create problems in particle counting in that the folds also feature a grey scale value similar to the particles. These are always light background dark ROI images. Plot Profile readily shows the issue to be resolved but, I do not know how to approach it. The irregular shape the folds acquire add to the challenge. The particles often gather in or near these folds so, if I could determine a means to handle this situation it would be useful. Even if this became a partial solution.
Thresholded_FoldedCoating.tif (4.3 MB)