Hello. I have recorded a movie (calcium imaging). From this movie I want to label individual cell (one at a time) and to build intensity plot for each time frame. How is it possible to do?
Pnevmatikakis et al. propose a complete Matlab pipeline for large scale calcium imaging data analysis in this paper:
You can find the Matlab source here:
I’m not aware of a similar solution using ImageJ, but would be happy to see alternative approaches.