I am trying to quantify small vesicles in IF images (see attached) which are very tiny dots in the red channel. The cells in this image are fibroblasts, so using the cell detection won’t work, as the detection won’t recognise the full length of the cell. In the image I also have stain in the green channel for the cytoskeleton, which gives me the shape of the cell.
I’ve tried using the pixel classifier and the object classifier, but neither gave me accurate results. Maybe I’m not using the correct settings? Or is there a different way I can use QuPath for this?
Thanks a lot!