I’m a new user of ImageJ, and I’m interested in measuring the fluorescence intensity of the mycelium in a transformed isolated (GFP) which is infecting plant tissue. I’ve read a lot of ImageJ articles, but I’m a little confused about how to do that. I was trying to adjust the threshold in order to obtain the measure but my results vary a lot, so, I read an article about segmentation and I tried to use statistical region merging and robust automatic threshold selection but I’m not sure if this could work for my pictures because the results are very different between them so I would like to know which is better to eliminate any bias in thresholding or please tell me if you have another suggestion
I’ve included a sample image below of the fluorescence I’m hoping to measure. I’d like to measure the fluorescence intensity of the mycelium (green)
Thank you in advance for your assistance!