How to quantify cytoskeleton density

Hello everyone,
I am trying to quantify actin density from a few confocal images. I came across this paper where they ‘skeletonize’ the images and quantify the actin density by using the following formula.

where n_{MF} and n_{Cell} are the pixel numbers constituting the skeletonized MFs and cell region respectively

I am just struggling to get the n_{MF} here. Anyone here can help me with this? Here is the link to the original paper -

Many thanks!

All good, managed to figure this out with another method :slight_smile:

Hi Praveen

For the benefit of the other forum members who might get into a similar situation, can you post a brief summary of how you resolved the problem.


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