How to process .nd2 files

cellprofiler
nd2

#1

Hi,
recently i had to take .nd2 picture with channel FITC and DAPI even tough i have zero experience in processing these type of files. I tried to search on the forum to learn the rope about it, but couldn’t find much.
Specifically, these images are myotubes in fitc and nuclei in DAPI.
Before i managed to process this kind of images when in tiff format and split.
Someone can explain to me the basics to .nd2 files processing?
Thank you in advance.


Cxd Movie analysis - thousands of cells over hundreds of images
#2

Hi,
You should be able to just drag and drop your images and then extract the channels from the metadata- set “Metadata”'s “Extract metadata?” to “Yes”, say “Extract from image file headers”, then hit “Update metadata”.


#3

thank you for the answer!