How to prevent background in images from being counted as objects?

Hello! I am using Cell Profiler to collect cell counts on a series of auto-fluorescent images. The pipeline I’ve used works well for the majority of them, but there are still others where the cell count is extremely high due to counting background regions as objects. I was wondering if there is any way to adjust this so the readouts can be more accurate. I’ll attach my current pipeline and some sample images below for reference. Thanks!

july10_pipeline_adjusted.cpproj (431.4 KB) ROI_C3_1_cropped.tif (2.6 MB) ROI_C3_2_cropped.tif (2.6 MB) ROI_C3_3_cropped.tif (2.2 MB)