I am trying to write a script that will batch process a lot of TIFF images (split the color channels, measure the densitometry of each channel, save the measures). Some of my images have two color channels and some have three, and I can’t figure out how to get the script to process the third window (if it exists), and skip on to finish the processing if there is no third window. Here is the code I have so far:
// Select the folder containing your images
dir1 = getDirectory("Choose Source Directory ");
list = getFileList(dir1);
// Select or create output folder
Output = getDirectory("Choose the output folder ");
//setBatchMode(true);
//delineate batch processing
for (k = 0; k<list.length; k++) { //list length is as many images as there are in the folder
showProgress(k+1, list.length);
open(dir1+list[k]); //open the first image (and then the next etc, after running)
imageName = getTitle(); //set “imageName” as the original name of each image
run("Split Channels");
selectWindow("C1-"+ imageName); //Select red (or Far Red) window
run("Measure"); // run "Measure" on red (or Far Red) window
selectWindow("C2-"+ imageName); //Select green (or blue) window
run("Measure"); // run "Measure" on green (or blue) window
if (File.exists("C3-"+ imageName)){ //If it exists, select blue window
(selectWindow("C3-"+ imageName))
run("Measure"); // if it exists, run "measure" on blue window
}
outName = imageName + "dens"; //still appends "dens" to ".tif" instead of file name before ".tif"
saveAs("Results", Output + outName); //Save each measure into Output folder separately for each image
close("Results"); // close Measure window
while(nImages>0)close(); // close all open images
} // still stops after CIE? --> doesn’t do all images in the folder, only 1/3
Error 1: When I unnest the “if” statement, instead of running “Measure” on C3 window, it runs it twice on C2 window. It used to not run a third measure at all – it just ignored the “if” statement
about C3 when I had the “if” commands nested. Now it runs the third measure,
but on C2 instead of C3.
Error2: Stops 1/3 of the way through the files in the folder, says it can’t find
the file or the file is not compatible. File name is “‘Folder I’m working in’/deskstop.ini”
Error3: Adds “dens” to the end of the file name after “.tif” instead of before “.tif”