This is my first time posting on here, but I am a bit stumped as to how I should do this. I am trying to do quantification of the protocerebral bridge, using Dapi and GFP at 2 micrometers thick slices. The problem is that I am trying to normalize the GFP to the Dapi, and I have not done this before. I have MaxStacks created, but since I have not done this before, I am trying to figure out how to do this. Can anyone help me? I’d very much appreciate it.