How to measure velocity, run length and step size of myosin VI using ImageJ

Hi everyone.

I am working on tracking the movement of myosin VI motor protein in living cell.
This is the video recording of Myosin VI that I acquired Myosin VI movement.tif (177.8 KB)
I would like to calculate some essential properties of myosin VI movement such as run length, speed, step size using ImageJ or Fiji plugin. I did a research and found that the plugin MTrack J and SpotTracker2D can do that, but I don’t know the right way to work with them. I hope that I can receive the advice from you guys. I really appreciate it.