I am new to ImageJ / Fiji and no native English speaker so my humble apologies in advance.
I am looking for a solution to the following task: I want the values of the pixels in different ROIs in the same photo, preferably in a list or so, to quantify the light intensity in the respective ROI in the picture.
The idea is to be able to quantify the amount of light transmitting a certain material via color photographies. To do so I took photos in a raw format and exported them to a composite 16-bit TIF, with respective values for the RGB channels to analyze it.
What I am looking for specifically is a way to extract the pixel values for each channel and the channels combined to be able to estimate the light intensity in certain ROIs to be able to further process the data.
As I am a new user I unfortunately can not upload an image, also they are rather large (about 140mb), so if anybody has a suggestion how to further illustrate it with a sample picture please let me know.
Thank you all in advance!