How to measure ratio intensity in live imaging

Hello everybody,

I am trying to measure the cytoplasmic and nuclear intensity for a specific channel in some images. Here is my pipeline 201807_CN_ratio_meas.cppipe (21.6 KB)

I am not sure if this is a good pipeline for what I need, especially for what concerns the way the cell is computed as a secondary object… Is there anybody who could suggest me a good way to analyze these cells?