Thank you for the quick respond. My goal is to measure the fiber size of different type muscle fiber (TypeIIA, TypeIIB, Non-typeIIAIIB).
Initially, I could use TypeIIA to calculate the fiber area of both TypeIIA (green) and Type IIB (blue), but the Type IIB includes Non-TypeIIAIIB fiber (Dark fiber). I upload the annotated image for non-typeIIAIIB fibers.
I’m trying to figure out a way to separate 3 different fiber types and measure their fiber area. The non-typeIIAIIB could include type I and typeIIX, but my lab is limited to measure those. In other words, using two channels to distinguish the 3rd channel.
Here, I also upload my pipeline screenshot and ClassifyObject image.