Hi,is there any way to define a region of interest in which I can measure the medium intensity of a certain channel which has the shape of a ring / doughnut?
If I identify nuclei, then expand the regions round the nuclei by distance - N, the result is a bigger region which includes the whole area of the nucleus.
What I would like to have instead, is just the ring between the nucleus and the outer region.
I would be really thankful if you could give me a hint.
Have a nice weekend!