I’m using “Plot Profile” and “Curve Fitting” inside Analyze menu to measure FWHM of tubulin in superresolution images. I’ve tried drawing and rotating a rectangle, but the box message “Line or rectangular selection required” appeared. Some publications show the rotated rectangles of the measurement areas which aligns the length of tubulins. I would like to know the accurate method to measure FWHM.
The following photo is a rectangle of the measurement area.