Is there anyone I could have quick online support while learning how to use Fiji software?
I’m trying to measure the distribution of lysosomes after I knocked down some gene in the cells. So what I need to quantify is the distance from each lysosome to the nucleus. I stained lysosome with LysoTracker and nucleus with DAPI, and the images were taken use Zeiss 710 confocal.
I know how to identify lysosomes by the size in Fiji. And I have read some paper which has measured lysosome distance to the nucleus, but I can’t find any guideline of how to do the measurement.
I would really appreciate the help, and I can also talk on whatssap/ messanger if it’s too complicated to explain.