In order to perform a mathematical calculation in image processing, you need the ability to perform mathematical operations on your images. So for example, if you wanted to take the logarithm of an image or the ratio of a couple of images, ImageMath allows you to do so.
[quote=“mahmoud”]thirdly , i have used the pipeline but cell profiler analyst showed me an error message i think it may be from SQL,
kindly look for the attached picture what is solve(ifile.it/skn3a8y/error.GIF)[/quote]
The error is generated because the properties file does not some of the fields correctly set, in this case, the database type; I wouldn’t be able to tell you more details without seeing the pipeline that generated the file. Even so, while we try to intelligently fill in as much of the settings in the properties file as we can, there are additional settings that would need to be filled in or checked manually. Your best course of action would be to take a look at the CPA manual, available from this page. However, based on the description of your project, you might be better served by using ExportToSpreadsheet in your pipeline and analyzing the output data in Excel or a similar package.
While we plan on integrating CellProfiler and CellProfiler Analyst in the future, the simple answer is that they do very different things; see question #2 in our FAQ.
As it turns out, there is not. However, we suggest making use of the example and tutorial webpages to help get started.