I am comparing the object identification function with Cellomics’ results. I managed to save a copy of cellomics image with identified object outlined in blue color. Ideally, I’d like to read in that color image, and overlay nuclei outline identified by CellProfier on top of it. However, the cellomics images I read in using “loadimages” module were automatically converted to gray, which makes the cellomics outline too dark to be seen. Is there anyway to read it in as a color image, overlay another layer of outline (generated by cellprofiler) and save the image as a color image as well?
Thanks so much!