I’m trying to analyze the porous structure of the bone, using the “Analyze particles” function. I want to analyze only the region within the boundary of the bone, in order to get data on the distribution and shape of the porous channels, as well as the ratio of porosity on the bone cross-section surface.
I know I should use the ROI manager, but I cannot find a way to link the ROI manager to the “Analyze particles” function. Within the ROI manager, I can only see the “measure” function, which doesn’t segment the porous, particle-like structures.
I have to mention that I’m working with stacks, and my ROIs are changing from slide to slide.
I will be grateful for the guidelines.