As I’m presently getting to grips with the execution of a pipeline to identify metaphase cells from brightfield images (I’m aware this is a somewhat difficult task).
I would greatly appreciate if anyone has attempted this or would like to make any suggestions on the pipeline construction.
The metaphases are the rounded cells with the halo’s, ideally the ones where the separating chromosomes are visible see e.g. .
And here are some images which could be used to run test this out.
I’ve also included what I consider to be positives… these are identifiable by the middle stripe.
I’ve my own ideas and a pipeline I’m working on, but before I post that I’m interested to see what others might come up with.
Many thanks in advance,
G144-G144D7_D2_1_2010y04m20d_11h30m.zip (4.17 MB)