when analysing quite dense tissues as spleen or tonsil we find it hard to set parameters for cell detection. We have been playing around with nucleus parameters and ended up setting background radius up to 8 , keeping median filter radius at 0 and setting Sigma to 1.3. Results differ a lot, depending on these parameter values, of course. What could we consider to improve our results, when cell groups are just no be separated into single cells? I attached an example which only 3 groups of cells(more included) .