How to get the portion of pixels in a ROI over certain threshold in a single channel in a composite z stack image?

imagej
roi
thresholding

#1

Hi,

I wonder how I can get the portion or number of pixels in a ROI over certain threshold, in a single channel in a composite z stack image? (zeiss .czi file)

When I try to threshold by Image>Adjust>Threshold, I can adjust nothing in the popup window and nothing changes. Maybe it’s because I am working on a composite z-stack file?

Anyone knows a simple solution to my question?

Thank you.


#2

Use the channel histogram.