Hi,

I’m trying to generate a theoretical PSF for deconvolution of 2D images. Most of the tools I have seen (e.g. PSFGenerator plugin from @daniel.sage) seem to generate 3D PSFs.

So my current thinking is to generate a 3D PSF with a Z-depth approximating the depth of field for the given NA/magnification and then take a sum/mean Z-projection of the PSF for 2D deconvolution.

Is that a valid approach or is anyone aware of a tool that directly models PSFs for 2D deconvolution?

Tagging

@bnorthan