I would like to filter based on the cytoplasmic intensity, then measure various things about the cells and nuclei associated with the filtered cytoplasms.
For example, if I have a cytoplasmic GFP marker, I want to know the cell area and DAPI intensity of the GFP+ cells. Or I might want to filter cells by area and then measure the nuclear intensity of the largest cells.
I can filter by the cytoplasm intensity, but then how do I recover the information about the associated nuclei and cell shape?
I tried to use RelateObjects but this didn’t seem to work - it told me how many nuclei I had but not their intensities.
Am I missing something obvious?