I am trying to extract and then be able to measure in 3D the space between two different membranes labelled with different colours. I have a z-stack acquired with a Lightsheet microscope, and I have managed to clean every channel so that when I threshold each channel I just obtain the membranes. I would like to know if there is a way I can extract the information of the layer between the membranes, since I am interested in measuring it.
Find attached a slice of a stack where you can appreciate how the two membranes look like.
TIFF_color3_Comp-236.tif (3.1 MB)
All the help is really appreciated!
Thanks in advance.