I need to calculate the curvature of a fluorescently labeled membrane for all points on its periphery. (Please take a look at the image below).
I found Kappa plugin in Fiji that may meet this purpose but I have a problem using it.
I imported my ND2 image-series data and selected my region of interest manually, and the spline curve appeared on what I drew manually, while I expected it to detect the boundaries of membrane inside that region.
Does this plugin capable of doing so?
If not, I have the boundaries of my data from my costume-made Matlab code; Can I import the boundaries from matlab, then implement Kappa algorithm on it?
Does anyone have any suggestions?
Thanks in advance for any help you cab provide.
PS. I guess it may help to mention @hadim (through wandering into the forum! ).